Neoadjuvant transcatheter arterial chemoembolization and systemic chemotherapy f

Min He, Jia-Bin Cai, Can Lai,Jun-Qing Mao, Jie-Ni Xiong, Zhong-Hai Guan, Lin-Jie Li, Qiang Shu Mei-Dan Ying,Jin-Hu Wang

Abstract

Key Words: Undifferentiated embryonal sarcoma of the liver; Unresectable; Neoadjuvant therapy; Transcatheter arterial chemoembolization; Systemic chemotherapy

lNTRODUCTlON

Undifferentiated embryonal sarcoma of the liver (UESL) is a rare and aggressive mesenchymal tumor that occurs mainly between the ages of 6 and 10 years, without gender predominance[1]. The prognosis of UESL has previously been considered poor[2]. Modern multimodal treatment and supportive therapy have improved survival[3]. Complete tumor excision plays the central role in the treatment of UESL[4]. Transcatheter arterial chemoembolization (TACE) is an effective therapeutic method and has been widely used in the treatment of patients with hepatic malignancy[5,6]. In an attempt to increase resection rate and improve the outcome of UESL, we performed preoperative TACE combined with systematic chemotherapy for the treatment of unresectable UESL in children and evaluated its feasibility and effectiveness.

MATERlALS AND METHODS

Patient characteristics

All the procedures in this study conformed to the ethical requirements of the Ethics Committee of the Children’s Hospital, Zhejiang University School of Medicine and have been approved. The children’s families provided signed informed consent. Six patients with UESL received neoadjuvant therapy (NAT) at our hospital between January 2004 and December 2019. The patients were 4 boys and 2 girls with a mean age of 7 years (range, 3-11 years). The symptoms at admission included abdominal pain, abdominal mass and jaundice. The longest tumor ranged from 8.6 to 14.8 cm (mean, 12 cm). One patient presented with pulmonary metastasis and another with retroperitoneal lymph node metastases. The details of the six patients are summarized in Table 1.

NAT

Pathological diagnosis was obtained by percutaneous needle biopsy before treatment in all patients. NAT included preoperative TACE and systemic chemotherapy. TACE was performed under general and sacral block anesthesia. With continuous monitoring, including electrocardiography, blood pressure and oxygen saturation measurements, the femoral artery was catheterized using the Seldinger technique. A 5-F Pigtail catheter was introduced into the abdominal aorta to perform aortography and define the main feeding artery of the tumor, and then a 4-F or 5-F Cobra catheter was advanced toward the tumor-feeding arteries for chemoembolization. The chemoembolic emulsion consisted of pirarubicin 40 mg/m2, vindesine 3 mg/m2, cisplatin 90 mg/m2and iodized oil 0.5 mLpermaximum tumor diameter (cm), which has been proved to have good efficacy and safety in the treatment of UESL and other tumors in our center[5,7]. The drugs were mixed and diluted in 120-180 mL of normal saline, and infused into the feeding artery at a rate of 120 mL/h. After perfusion, the outline of the entire tumor was revealed (Figure 1).

Intravenous chemotherapy was administered 3 wk after TACE if the blood parameters were at normal levels. The chemotherapy regimen consisted of vindesine 3 mg/m2on day 1, carboplatin 150 mg/m2and pirarubicin 20 mg/m2on days 2 and 3. Vindesine was injected every 7 d.

Tumor response was evaluated by enhanced computed tomography (CT) scan or magnetic resonance imaging after every cycle of NAT according to the revised Response Evaluation Criteria In Solid Tumors (RECIST) version 1.1[8]. Surgical resection was carried out after one or two cycles depending on the patient’s response. Postoperative intravenous chemotherapy was given every 3 wk, and the drugs administered were those listed above.

Toxicity evaluation

Toxicity was evaluated after each session of NAT. Cardiac function was monitored by echocardiography and ECG; renal function by serum electrolytes, urea, creatinine, and creatinine clearance; and liver function by aspartate aminotransferase and alanine aminotransferase (ALT). Complete blood cell count was repeated weekly to assess bone marrow suppression.

Follow-up

All patients were followed every 2 mo during the first year after comprehensive treatment, every 3 mo during the second and third years, every 6 mo during the fourth and fifth years and annually after 5 years. Follow-up examinations included physical check-up, imaging (abdominal ultrasound and CT, chest X-ray or CT), and laboratory testing (blood and urine analysis, and liver and renal function tests).

RESULTS

TACE was successfully performed in all patients. The tumor volumes decreased by 18.2%-68.7%, with a mean decrease of 36% after 1 cycle of NAT (t= 3.524,P= 0.017). According to the RECIST criteria, 4 patients had a partial response (PR) and underwent surgery, while 2 had stable disease (SD) and received another cycle of NAT before surgery. Retroperitoneal lymph node metastasis and pulmonary metastasis observed in one patient, respectively, were slightly reduced after TACE, and no regrow or other lesions appeared before surgery. Total tumor resection was performed in all patients without positive surgical margins and major postoperative complications. Retroperitoneal lymph node dissection was performed in the patient with lymph node metastasis. The patient with lung metastasis was not treated surgically as the lesions were no longer obvious after chemotherapy. Massive tumor necrosis was seen on pathological examination of the surgical specimen: ＞ 90% necrosis in 2, ＞ 50% necrosis in 3, and 25% necrosis in 1, with an average of 71.8%. Six to 10 courses of regular venous chemotherapy were administered after surgery in patients without metastasis, and 12 courses in the 2 patients with metastases.

The toxic side effects included fever (37.8-39.9 °C) in 5 cases, nausea and vomiting in 3, and mild bone marrow suppression in 3, all these symptoms recovered within 1-2 wk following symptomatic treatment. All patients showed varying degrees of ALT elevation, which returned to normal within 7-10 d after treatment with hepatoprotective drugs. No cardiac or renal toxicity, bleeding or nontarget embolization occurred.

Table 1 Clinicpathological data, treatment, follow-up and outcome of 6 patients with undifferentiated embryonal sarcoma of the liver

Figure 1 Transcatheter arterial chemoembolization procedure of case 6. A: The catheter was introduced into the tumor-feeding artery and performed angiography, showing the stereoscopic configuration of "holding ball"; B: After injection of anticarcinogen and lipiodol, the outline of the entire tumor was revealed.

The follow-up duration ranged from 2 to 15 years (median 8 years), and the follow-up rate was 100%. At the last follow-up on February 1, 2021, all patients were alive and disease-free.

DlSCUSSlON

Early detection of UESL is difficult, and it is usually found when the tumor becomes larger or metastasizes. It metastasizes in up to 15% of children, usually to the lungs, pleura and peritoneum[9]. Preoperative chemotherapy is currently recommended if a huge unresectable tumor or metastases are present. Although intravenous chemotherapy, as the mainstay of adjuvant treatment for UESL, has improved survival rates, the toxicity induced by systemic chemotherapy may result in early death or potential long-term and late effects[10,11]. In addition, the therapeutic effects in UESL with incomplete resection or distant metastases are still unsatisfactory[12].

TACE was first introduced in the treatment of adult liver tumor in the late 1970s and yielded good results[13]. Since then, reports of TACE in pediatric cases of hepatoblastoma have gradually increased[6,14,15]. In previous studies at our center, preoperative TACE was effective in the treatment of UESL in two cases[5]. Also, neoadjuvant TACE and systemic chemotherapy have been administered in patients with Wilms tumor and clear cell sarcoma of the kidney and achieved satisfactory results[7,16].

Complete tumor excision plays a central role in the treatment of UESL[4]. TACE can increase the total resection rate in several ways. TACE can deliver a higher concentration of anticancer drugs into the tumorviadirect perfusion through the tumor-feeding artery, which reduces the dose of drugs and systemic toxicity[5,6,16]. Lipiodol embolization can reduce the blood supply of tumor and accelerate necrosis. High concentrations of chemotherapeutic drugs and internal ischemia cause the tumor to gradually shrink. After TACE, a pseudo-capsule can be seen around the tumor and a boundary exists between the tumor and normal liver tissue during the operation (Figure 2). These are conducive to complete resection, reducing the rate of positive surgical margins, and diminishing intraoperative bleeding (Figure 3). In this study, all the patients achieved total resection without positive surgical margins and severe bleeding.

TACE plays an important role in the primary lesion but has little effect on distal metastasis[15,17]. In this series, two patients had pulmonary and retroperitoneal lymph node metastasis on admission, respectively. Fortunately, after TACE and before systemic chemotherapy, we found that the metastasis was slightly reduced and no other lesions appeared. Following infusion into the hepatic artery, chemotherapy drugs will still flow through the hepatic vein to the heart and the entire body; thus, the extrahepatic lesions can also be suppressed. However, intravenous chemotherapy is still essential, as the dose of chemotherapy drugs flowing into the body following TACE is limited, and do not achieve an effective concentration. Preoperative intravenous chemotherapy can effectively inhibit primary hepatic and extrahepatic lesions, while postoperative intravenous chemotherapy can reduce the risk of recurrence and improve the survival rate[18,19]. Therefore, systemic chemotherapy can complement TACE. In our series, prolonged postoperative chemotherapy was performed in the two patients who are presently recurrence-free. However, the prognosis is poor, even if the patient receives intravenous chemotherapy, when complete excision of the tumor cannot be achieved[4]. The patient with lung metastases who is currently event-free still requires long-term follow-up.

In recent years drug-eluting beads (DEBs) have been introduced as novel drug-delivery agents for TACE, which can deliver higher concentrations of drugs to the tumor and lower systemic concentrations[20]. Studies have shown a lower incidence of systemic toxicity and improved tolerance with DEBTACE, but overall survival did not differ from conventional lipiodol-TACE[21,22]. DEB-TACE has been widely used for the treatment of hepatocellular carcinoma in adults, but has not been reported in pediatric liver malignancies, and its clinical safety needs to be confirmed by further clinical trials in children. In addition, DEB-TACE reduces systemic complications by lowering extrahepatic drug concentrations, but it also decreases the inhibition of extrahepatic lesions. Therefore, DEB-TACE was not used in this study. In the near future, DEB-TACE is expected to be applied in the treatment of unresectable intrahepatic malignancy in pediatric patients.

No technical complications including bleeding, vascular injury, or nontarget embolization were noted in this study. Nontarget embolization must be viewed as a critical situation during the TACE procedure, as its appearance may lead to serious consequences[23]. The incidence of nontargeted organ embolization, such as pulmonary embolism, splenic infarction, and gallbladder infarction, was found to be approximately 5%[23,24]. The main risk factors for the development of these complications include: (1) Non-selective placement of the catheter; (2) High doses of cytostatics and iodized oil used in TACE; and (3) Use of small-diameter embolic agents[24]. A case of paraplegia after TACE in a patient with clear cell sarcoma of the kidney was reported in our center, which was caused by injection of chemotherapeutic drugs and lipiodol into the spinal cord through the lumbar artery. Hence, angiography must be performed repeatedly to confirm the location of the catheter during the TACE procedure especially before injection of the embolic agent.

Postembolization syndrome and drug toxicity mainly include fever, abdominal pain, gastrointestinal reaction (nausea and vomiting), myelosuppression and organ damage (cardiotoxicity, renal insufficiency, and hepatic dysfunction)[25]. In our cases, fever was the most common symptom after TACE, possibly due to massive necrosis of the large tumor, which lasted a long time and did not respond to antibiotics. Under such circumstances, short-term small doses of hormones can be used to suppress inflammation and lower prolonged fever. Elevated ALT levels occurred in all of our patients, which returned to normal within 7-10 d after treatment without affecting follow-up treatment. Renal failure, cardiac damage, and severe myelosuppression did not occur. Hence, NAT may be considered a safe preoperative treatment for UESL.

Figure 2 The imaging manifestation of case 6. A and B: Computed tomography scans showed a giant mass of the right liver (diameter 14.8 cm); C and D: One month after neoadjuvant therapy: The tumor volumes shrunk by 48.8%, lipiodol deposits in the tumor with a clear boundary; E and F: One month after tumor resection.

Figure 3 These are conducive to complete resection, reducing the rate of positive surgical margins, and diminishing intraoperative bleeding. A: The gross of tumor appears as border clear with about 10 cm in diameter; B: The cut surface is red, brown or yellow and has soft qualitative with focal necrosis.

It is noteworthy that two of our patients underwent 2 cycles of NAT before surgery. They achieved SD (tumor shrinkage was approximately 20%) after one cycle of NAT, and still had large tumors with PRETEXT stage III on radiography[26]. After another cycle of NAT, PRs (tumor shrinkage of approximately 35%) were achieved. Therefore, it is suggested that patients who are insensitive to the initial response should be considered for a second round of NAT, which can yield better results. However, unlimited chemotherapy is not desirable. Generally, surgery should be performed after two cycles of NAT, and liver transplantation should be considered if a limited response occurs in a patient with an unresectable tumor.

There were some limitations in this study. Firstly, the data were from a single institution and based on a retrospective analysis. In addition, there was no control group due to the small number of cases, and the assessment of disease response cannot definitely be attributed to TACE or systemic chemotherapy. Further prospective and controlled studies with a larger cohort are needed to compare the efficacy of TACE, systemic chemotherapy and NAT for UESL, respectively.

CONCLUSlON

The present study indicated that the use of NAT effectively reduced tumor volume, cleared tumor margins, and caused massive tumor necrosis. This chemotherapy regimen may be a promising choice for successful surgery of UESL in children.

ARTlCLE HlGHLlGHTS

FOOTNOTES

Author contributions:All authors contributed equally to this work; Wang JH was the guarantor and designed the study; He M drafted the initial manuscript; Wang JH and Ying MD helped to review the literature; Lai C and Wang JH conducted the TACE process; Mao JQ and Li LJ were the oncologists who treated the patient; Wang JH, He M, Cai JB, Xiong JN, Guan ZH and Shu Q were the surgeons who treated the patient; all authors issued final approval for the version to submitted.

Supported byYouth Program of Natural Science Foundation of Zhejiang Province, No. LQ20H160027; and National Natural Science Foundation of China, No. U20A20137.

lnstitutional review board statement:The study was reviewed and approved by the Children’s Hospital, Zhejiang University School of Medicine Institutional Review Board, No. 2021-IRB-252.

lnformed consent statement:All study participants, or their legal guardian, provided informed written consent prior to study enrollment.

美国Waters公司MICROMASS Quattro Micro API型质谱仪，配有电喷雾离子化源（ESI），数据采集：MassLynx 4.1软件（美国Waters公司）。岛津LC‐20 10CHT高效液相色谱仪，恒温水浴箱（Heto Holten Denmark），匹配紫外检测器、荧光检测器。

Conflict-of-interest statement:All the authors report no relevant conflicts of interest for this article.

Data sharing statement:No additional data are available.

Open-Access:This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BYNC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is noncommercial. See: https://creativecommons.org/Licenses/by-nc/4.0/

Country/Territory of origin:China

ORClD number:Min He 0000-0003-0178-097X; Jia-Bin Cai 0000-0002-2233-0022; Can Lai 0000-0002-4474-3987; Jun-Qing Mao 0000-0002-3162-9106; Jie-Ni Xiong 0000-0003-1603-1728; Zhong-Hai Guan 0000-0002-8397-4585; Lin-Jie Li 0000-0001-7311-7283; Qiang Shu 0000-0002-4106-6255; Mei-Dan Ying 0000-0003-0039-9683; Jin-Hu Wang 0000-0002-7749-2475.

S-Editor:Fan JR

L-Editor:A

P-Editor:Fan JR