Cryptococcus infection with asymptomatic diffuse pulmonary disease in an immunoc

Yong Li, Lei Fang, Fang-Qun Chang, Fang-Zhou Xu, Yan-Bei Zhang

Yong Li, Lei Fang, Fang-Qun Chang, Fang-Zhou Xu, Yan-Bei Zhang, Department of Geriatric Respiratory and Critical Care, The First Affiliated Hospital of Anhui Medical University, Hefei 230000, Anhui Province, China

Yong Li, Department of Tuberculosis, Anhui Medical University Clinical College of Chest &Anhui Chest Hospital, Hefei 230000, Anhui Province, China

Abstract BACKGROUND Cryptococcus presenting as an opportunistic pathogen mainly affects immunocompromised patients, but the disseminated form of infection is rare among immunocompetent populations. The partial radiographic characteristics of pulmonary cryptococcosis mimic lung carcinoma, leading to unnecessary open chest exploratory surgery, and the lack of a gold-standard noninvasive diagnostic increases the risk of misdiagnosis. Positron emission tomography/computed tomography (PET/CT), a sensitive method for distinguishing malignant tumors,coupled with cryptococcal latex agglutination test showing a high positive rate may overcome these issues.CASE A 36-year-old man presented for general examination, without health complaints.Routine CT showed multiple pulmonary nodules and a mass with high maximum standardized uptake value. Initially, we suspected primary malignancy with hematogenous metastasis. Although his routine fungal analysis had been negative, subsequent CT-guided percutaneous core needle biopsy and histopathology examination indicated a diagnosis of pulmonary cryptococcosis.Fluconazole (200 mg/d) antifungal drug treatment was initiated, and 1 mo later the pulmonary mass had reduced in size markedly (on chest CT scan) without any complications.CONCLUSION Serologic and PET/CT examinations may not rule out cryptococcosis, and percutaneous lung puncture is critical under all circumstances.

Key Words: Cryptococcus; Immunocompetent; Positron emission tomography; Computed tomography; Cryptococcal latex agglutination test; Lung diseases; Fungal; Case report

lNTRODUCTlON

Cryptococcosis is a systemic infection caused by two species of the encapsulated yeastlike fungusCryptococcus:Cryptococcus gattii(C. gattii) andCryptococcus neoformans(C.neoforman)[1]. These opportunistic fungal pathogens usually cause cryptococcosis in immunocompromised patients, with the respiratory system being the usual route of infection. Infections in immunocompetent persons are much rarer.

Positron emission tomography/computed tomography (PET/CT) is a useful tool for differentiating benign and malignant tissues. Higher uptake values of fluorodeoxyglucose on PET/CT reflect the characteristics of a malignant disease. Unfortunately, since the radiographic characteristics of pulmonary cryptococcosis mimic lung carcinoma,clinicians may order open chest exploratory surgery, with patients bearing the burden of undergoing an unnecessary invasive procedure[2].

Cases of pulmonary cryptococcosis, therefore, require further noninvasive testing for a definitive diagnosis; yet, no gold-standard noninvasive diagnostic exists and the risk of misdiagnosis remains large. Prior to a biopsy and histological assessment [by Grocott's methenamine silver (GMS) staining], the most common suspected diagnoses are lung cancer, inflammatory granuloma, metastases from an extrathoracic malignancy, and pulmonary tuberculosis.

As described below for our case, CT-guided percutaneous core needle biopsy of a presumed lung carcinoma can be critical for a definitive diagnosis ofCryptococcusinfection.

CASE PRESENTATlON

Chief complaints

A 34-year-old apparently immunocompetent Chinese man presented for general health examination. On routine CT, multiple pulmonary nodules and a mass were incidentally found.

History of present illness

The patient reported having had no symptoms of discomfort prior to presentation,including cough, sputum, chest pain, chills, or fever. He also denied tiring easily,having night sweats, and rapid weight loss.

History of past illness

The patient reported occupation as an engineer, smoking ten cigarettes a day for the past 3 years, and having no pets. He also had no history of allergies, hepatitis B, or diabetes mellitus, or any other significant medical history.

Physical examination

Physical examination upon admission found bilateral lungs to be clear, with no wheezing, rales, or rhonchi detected by auscultation. Temperature was 36.5 °C. Heart rate was 95 beats/min. Respiratory rate was 20 breaths/min. Blood pressure was 104/71 mmHg. There were no other remarkable findings.

Laboratory examinations

Test findings for liver function, renal function, and electrolytes were normal. The values of fasting blood glucose were within the normal range twice during hospitalization. All inflammatory biomarkers were also within the normal range. The result of human immunodeficiency virus (HIV) serology test was negative. Tumor markers in blood, including carcinoembryonic antigen, progastrin-releasing peptide,neuron-specific enolase, and cytokeratin 19-fragments (CYFRA 21.1), were all normal.Serology test forCryptococcusantigen also gave negative results.

Imaging examinations

Due to the pulmonary lesions of unknown cause, PET/CT was arranged. The imaging showed multiple nodules in both lungs, enlarged right hilar lymph nodes, and an elliptic mass lesion without clear margin in the right lower lobe (Figure 1). The mass showed higher uptake values of18F-fluorodeoxyglucose (FDG) and SUVmax of 16.4 in normal scans and 19.1 in delayed scans. The SUVmax for FDG uptake of those nodules was 6.8 in normal scans and 10.2 in delayed scans. The two enlarged lymph nodes in the right hilum gave 4.8 in normal scans and 6.9 in delayed scans.

Further diagnostic work-up

Subsequently, the patient underwent CT-guided percutaneous core needle biopsy of the right lower-lobe mass. No fungi or remarkable bacteria were found in the puncture fluid smear.

MULTIDISCIPLINARY EXPERT CONSULTATION

Department of Pathology, Nanjing Gulou Hospital Affiliated to Medical School of Nanjing University

The pathologic consultation found granulomatous inflammation. GMS was positive and periodic acid-Schiff staining was negative (Figure 2). No evidence of malignancy was noted. In summary, a diagnosis of cryptococcal pneumonia was established.

FINAL DIAGNOSIS

A diagnosis of pulmonary cryptococcosis was made according to the radiologic evidence of pulmonary lesions and positive histopathology, although the serology test forCryptococcusantigen was negative.

TREATMENT

Fluconazole (200 mg) per day orally.

OUTCOME AND FOLLOW-UP

After 1 mo and 3 mo of treatment, the pulmonary lesions were found to be markedly reduced in size on chest CT (Figures 3 and 4).

Figure 1 Positron emission tomography/computed tomography showing bilateral lesions. An elliptic mass without clear margin was shown in the right lower lobe. The size of the mass was 3.46 cm × 2.39 cm. The enlarged right hilar lymph nodes showed a high value of SUVmax. There were also scattered nodules found in both lungs.

Figure 2 Pathological examination revealed Cryptococcus infection. A: Grocott's methenamine silver staining showed Cryptococcus spores by black staining (arrow); B: Periodic acid-Schiff staining was negative. Original magnification: × 400.

DISCUSSION

Pulmonary cryptococcosis is prevalent in patients with compromised cell-mediated immunity, such as those with HIV infection or who have been subject to chemotherapy or other immunosuppressive therapy[3,4]. It is known that patients with HIV infection are more likely to have central nervous system involvement with the cryptococcosis disease than those without. In that group, the disease itself is associated with a high mortality rate. Cryptococcosis can also occur in individuals with normal immunity and causes severe nervous system infections[5], but such cases are rare.Table 1 illustrates the special features of our report.

For our case, the patient was otherwise healthy and had no neurological symptoms.However, he presented bilaterally disseminated lesions in the lung, which is extremely rare for an asymptomatic immunocompetent person, and this increased the chance of mistaken and missed diagnosis. Interestingly, his cryptococcal latex agglutination test(CLAT) was also negative. In other reports in the literature, the positive rate for CLAT has been high, even exceeding 90%[6,7]. CLAT is also used to evaluate response to therapy for pulmonary cryptococcosis in patients without HIV infection[7].

Most cryptococcal strains are encapsulated by polysaccharides[8]and capsuledeficientC. neoformansmay have reduced virulence[9]. A cryptococcal strain that has lost its capsular material can, however, trigger strong inflammatory reactions, which tends to lead to the formation of granulomas composed of histiocytes, giant cells, and lymphocytes. This is consistent with our pathological outcome. We speculated that our patient may have been infected with a low-virulenceC. neoformansstrain, thus causingthe false negative CLAT result[10]. Certainly, other unknown possibilities cannot be excluded.

Of the twoCryptococcusspecies,C. neoformansis widespread, especially in pigeon feces or in soil contaminated with these feces, and it tends to cause disease in patients who are immunocompromised. In Taiwan, there is evidence to show a significant prevalence ofC. gattiiinfection associated with the eucalyptus tree[11].C. gattiiusually causes disease in immunocompetent persons and behaves as a primary pathogen. The percentage ofC. gattiiinfection in immunocompetent hosts is significantly higher than that ofC. neoformansinfection[12].

In 2018, the World Health Organization recommended that without the results of cerebrospinal fluid, rapid serum, plasma, or whole-blood cryptococcal antigen assays are the preferred diagnostic methods (either lateral flow assay or latex agglutination assay). Some studies have reported that CLAT has an insufficient sensitivity forC.gattiicompared to the lateral flow assay, increasing the risk of delayed diagnosis and probability of misdiagnosis[13]. As such, in order to diagnose cryptococcosis, multiple detection methods should be applied to improve the accuracy of diagnosis.

C. gattiiinfection is more common in tropical areas[11], which is inconsistent with the geography of our patient's residential area. During the subsequent visit, the patient told us that one of his neighbors kept pigeons, which supported our speculation that the patient was likely to have been infected with a low-virulence strain ofC.neoformans.

Figure 3 Chest computed tomography scan after 1 mo of antifungal treatment showing resolution of the bilateral lesions. The mass in the right lung was reduced markedly. A: The 25th floor scan; B: The 26th floor scan.

Figure 4 Chest computed tomography scan after 3 mo of antifungal treatment showing near complete disappearance of the nodules and infiltration distributed around the lesions. Only a small number of pulmonary cavities remained at this time. A: The 29th floor scan; B: The 30th floor scan.

The most common radiographic characteristics of pulmonary cryptococcosis consist of solitary pulmonary mass or nodules, combined patchy and nodular shadows,cavitation, and enlarged mediastinal lymph nodes[14]. However, the most common imaging finding of immunocompetent patients is single nodule. Mass-like and bronchopneumonic patterns are common in immunocompromised patients and rare in the patients with normal immunity[15]. PET/CT is a sensitive method for distinguishing malignant tumors from benign tissue, and it has gradually become a useful imaging technology for the differential diagnosis, staging, and follow-up of cancer[16]. The related SUVmax quantitatively measures the FDG uptake of a tumor.

Generally, the SUVmax value of 2.5 is used as the cut-off for differentiating benign lesions from malignant ones, as it is known to have the best sensitivity and specificity for such. However, the diagnostic accuracy of this value is not high in pulmonary infectious diseases, especially for those due to granulomatous diseases[17]such as in our patient. The SUVmax values for cases of pulmonary cryptococcosis may vary widely,indicating anywhere from mild to marked uptake. In previous reports, the SUVmax of the cryptococcal lesions fluctuate from 0.93 to 13.0[15,18], with most cases exceeding the 2.5 threshold. Nevertheless, few of the SUVmax values of pulmonary lesions reach 19.

The mechanism of increased FDG uptake in cryptococcal lesions may be related to the “respiratory burst”, which increases cellular glucose metabolism. Based on this principle, PET/CT can play a role in assessing the treatment of patients with fungal infection[19]. Therefore, diagnosis of malignant tumor by PET/CT should exclude the possibility of fungal infection. Our case was remarkable since we could not find evidence of immunocompromise in our patient; additional reports of such cases and bench-to-bedside studies will help to explain the development of masses and diffuse lesions in patients who are infected with a low-virulence strain (as we suspect our patient was).

CONCLUSION

We describe here an immunocompetent patient with pulmonaryCryptococcusinfection, who manifested a mass and diffuse lesions in his lung while being asymptomatic. Although it is very rare, cryptococcosis must be included in the differential diagnosis of pulmonary lesions in patients without immunosuppression. It is important to recognize that pulmonary fungal infection can mimic metastases, even when the patient has been diagnosed with lung cancer by PET/CT. Furthermore, the potential for false negative results of fungal smears and serologic testing for cryptococcosis is another risk for misdiagnosis of this infectious disease. Thus,percutaneous lung puncture is critical under all circumstances in order to promote more timely initiation of appropriate treatment and avoidance of unnecessary surgery.