CD56+ lymphoepithelioma-like carcinoma of the lung:A case report and literature

Lin Yang,Hua Liang,Li Liu,Lei Guo,Jian-Ming Ying,Su-Sheng Shi,Xing-Sheng Hu

Lin Yang,Li Liu,Lei Guo,Jian-Ming Ying,Su-Sheng Shi,Department of Pathology,National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100021,China

Hua Liang,Department of Medical Oncology,Qingdao Central Hospital,Qingdao 266042,Shandong Province,China

Xing-Sheng Hu,Department of Medical Oncology,National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100021,China

Abstract

Key words: Lymphoepithelioma-like carcinoma;Lung;CD56;EBER;Case report;Literature review

INTRODUCTION

Lymphoepithelioma-like carcinoma (LELC) is a relatively rare type of nonkeratinizing carcinoma characterized by prominent infiltration of lymphocytes.Although it primarily originates from the nasopharynx,rare cases occurring in other organs (e.g.,the liver,breast,bladder,cervix,and lung) have also been previously reported[1-5].Primary LELC of the lung was first described by Béginet al[6]in 1987.This disease accounts for merely 0.92% of lung cancer cases and mostly affects the Asian population.According to the 4th edition of the World Health Organization classification of thymic tumors,LELC belongs to the category of “other and unclassified carcinoma”[7].Owing to an insufficient number of cases,internationally recognized diagnostic criteria for LELC have not been established thus far.Misdiagnosis of LELC as adenocarcinoma or squamous carcinoma has been previously reported[8,9].Following the introduction of the concept of precision medicine,clinicians and researchers have performed extensive investigations on the molecular profile of patients with lung LELC to facilitate differential diagnosis and personalized treatment.Herein,we report a rare case of lung LELC with CD56 expression.In addition,we systematically review the epidemiological,clinical,and prognostic features of lung LELC documented in the literature to better understand this rare disease.

CASE PRESENTATION

Chief complaints

A 51-year-old man presented to Cancer Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College in May 2017 with complaints of cough,expectoration,and chest pain.

History of present illness

The symptoms included cough and expectoration lasting ＞ 2 mo and chest pain lasting 1 wk.

History of past illness

No past illnesses were documented.

Personal and family history

The patient was a non-smoker without a family history of cancer.

Physical examination

Positive tenderness of the sternum,spine,and left femur was found during physical examination and the numerical rating scale score was 8.

Laboratory examinations

The investigation of serum tumor markers showed that the cancer antigen 125,cytokeratin fragment antigen 21,and neuron-specific enolase were 37.7 U/mL,13.68 ng/mL,and 76.71 ng/mL,with the corresponding upper limit of normal of 35 U/mL,3.3 ng/mL,and 16.3 ng/mL,respectively.

Imaging examinations

Positron emission tomography-computed tomography (PET-CT) revealed the presence of a mass (maximum diameter:4.2 cm) in the right upper lobe,as well as enlarged lymph nodes and multiple metastases in the bilateral clavicles,mediastinum,and systemic skeleton (e.g.,the head,chest,vertebra,and pelvis).The mass was adjacent to the mediastinal pleura and indistinct from the mediastinal enlarged lymph nodes,as well as the right lung hilum;the largest standardized uptake value was 18.4(Figure 1A and B).Multiple bone metastases were illustrated through magnetic resonance imaging (Figure 1C).Pharyngorhinoscopy did not reveal abnormalities in the nasopharynx.

Further diagnostic work-up

Bronchoscopic biopsy showed that a highly extruded heterotypic cell mass was present in the mucosal tissue that was overlain by ciliated columnar epithelia;this mass was considered a small-cell lung cancer.Bronchoscopic biopsy combined with immunohistochemistry (IHC) indicated poorly differentiated cancer with obviously extruded cells.Local extruded regions showed large cells with visible nucleoli.The IHC results were as follows:AE1/AE3 (1+),thyroid transcription factor 1 (-),CD56(1+),chromaffin A (ChrA) (-),synaptophysin (Syn) (-),Ki-67 (+,＞ 75%),P63 (-),and P40 (-).Cervical lymph nodes were resected to reach a differential diagnosis.Microscopic examination showed that tumor cells were large with vesicular nuclei and prominent nucleoli,showing the typical morphological feature of LELC (Figure 2A and B).IHC staining demonstrated that the tumor cells were positive for AE1/AE3(3+),cytokeratin 18 (2+),CK5/6 (focus+),P40 (focus+),P63 (focus+),CD56 (3+),ChrA(-),Syn (-),CK7 (-),Napsin A (-),ALK-VentanaD5F3 (-),ALK-Neg (-),and Ki-67 (+＞ 75%) (Figure 2C).Encoded small nuclear RNA (EBER) was positive,suggesting infection with Epstein-Barr virus (EBV) (Figure 2D).There were no mutations detected in the epidermal growth factor receptor (EGFR) andKRASgenes.

FINAL DIAGNOSIS

The final diagnosis was CD56-positive pulmonary LELC.

选取2012年8月至2017年8月在河南科技大学第一附属医院确诊并行TURP的良性前列腺增生患者1126例，根据病例资料分为储尿期症状组和排尿期症状组。

TREATMENT

Prior to conducting the IHC staining,the EP regimen (cisplatin:50 mg days 1 and 2,40 mg day 3,intravenously guttae (ivgtt) /q21d + etoposide:200 mg days 1-2,100 mg day 3,ivgtt) was administered as first-line therapy since May 12,2017 for the relief of whole body pain upon request by the patient.

OUTCOME AND FOLLOW-UP

After completion of two cycles of chemotherapy,CT showed that the size of the mass was reduced (largest diameter:2.8 cm).Partial remission was achieved and whole body pain was obviously relieved with the reduction of the numerical rating scale to 2.After four cycles of chemotherapy,metastasis was observed in the level VI lymph nodes.Progression of disease was reported with a progression-free survival of 2.5 mo.

DISCUSSION

Primary LELC of the lung is a rare disease.A total of 138 articles were searched in PubMed using “lymphoepithelioma-like carcinoma” and “pulmonary” as key words to comprehensively summarize the epidemiological,clinical,and prognostic characteristics of this condition.The available literature mainly included retrospective studies and individual reports,and no case-control studies.A total of 458 cases in ten high-quality publications with complete survival information were collected and analyzed as follows.

Epidemiologically,primary lung LELC is a rare malignant tumor.Since its first report in 1987[6],approximately 500 cases have been reported[9-11].Approximately twothirds of cases were documented in Southeast Asia,including southern China,Hong Kong,Taiwan,and other regions.The incidence in males and females is similar and the average age at diagnosis is 54.4 years[12].Approximately 75% of patients with primary lung LELC were non-smokers,suggesting that this disease is not associated with smoking[10].It was reported that LELC is closely related to infection with EBV in the Asian population.The positive rate of EBV was 93.8% (30/32) in the Asian population compared with 0% (0/6) in the Western population[13,14].In this case,the 51-year-old patient with positive EBV status was a non-smoker.

Compared with non-small cell lung cancer (NSCLC),primary lung LELC is not characterized by special clinical manifestations.Of note,approximately 40% of the cases were asymptomatic.Dry cough,hemoptysis,chest pain,dyspnea,and other chest discomfort were the most commonly reported symptoms,while fever and weight loss were rare.Chest CT is the first choice for further examination.The mass of LELC was large in size and located near the mediastinum;some of the mass presented a tendency for vascular encasement[15,16].PET-CT is another option for examination.The sensitivity of PET-CT in the diagnosis of lung LELC is 92.3% (12/13 cases),and its specificity is 66.7% (4/6 cases)[17].In the present case,the patient presented to our hospital with cough and chest pain lasting ＞ 2 mo and 1 wk,respectively.PET-CT revealed a mass adjacent to the mediastinal pleura in the right upper lobe,with a maximum diameter of 4.2 cm.

In terms of pathological characteristics and differential diagnosis,pulmonary LELC has a similar morphology to nasopharyngeal LELC.Microscopic observation revealed larger tumor cells with nest-like or syncytial distribution,slightly stained cytoplasm,and vesicular nuclei with eosinophilic prominent nucleoli.Pathological mitosis is common,and focal squamous and spindle cell differentiation can occur[18].The IHC analysis showed that the tumor cells were primarily positive for CK5/6 and P63,with positive rates of 100% (11/11 cases) and 94% (15/16 cases),respectively[10].The most important differential diagnosis of lung LELC is nasopharyngeal LELC with lung metastasis,owing to their almost identical morphologies.Clinical history and nasopharyngeal biopsy are helpful in differential diagnosis.In our case,pharyngorhinoscopy did not reveal abnormalities in the nasopharynx.The cell morphology and lymphocytic interstitial background were typical.IHC showed that CK5/6 and P63 were focally positive,supporting the diagnosis of LELC.However,CD56 showed strong positivity in this case.Therefore,the differentiation between LELC and large cell neuroendocrine carcinoma (LCNEC) was the most challenging task in the present case.

LCNEC exhibits a neuroendocrine morphology,such as organoid nesting,trabecular growth,rosette-like structures,and peripheral palisading patterns.Solid nests with multiple rosette-like structures forming cribriform patterns are common.The tumor cells are generally large,with lightly-stained cytoplasm and prominent nucleoli.In addition,one or more indicators among ChrA,Syn,and CD56 are positive.CD56 showed lower specificity for neuroendocrine differentiation in lung cancer;however,it is the most sensitive marker in certain morphological context[19].ChrA and Syn are the most reliable markers in distinguishing LCNEC from nonneuroendocrine tumors.Positive staining for either ChrA or Syn may be sufficient[20-23].In our case,only CD56 was positive,whereas the other two endocrine markers were negative.The diagnosis of LCNEC was excluded considering the positivity for CD56 according to the 2015 definition of the World Health Organization[7]and the enhanced expression of EBER.In the literature,Jianget al[24]previously reported a case of CD56-positive LELC.The present case was the second reported lung LELC with expression of CD56.

The genetic profile of lung LELC is very similar to that of EBV-associated tumors,such as nasopharyngeal carcinoma and natural killer/T-cell lymphoma.In contrast,it is markedly different from other primary lung tumors.The tumor protein p53 (TP53)mutation is the most frequent mutation in lung LELC (19.5%,8/41 cases) and TNF receptor associated factor 3 (TRAF3) exhibits a high deletion rate (53.7%,22/41 cases);these alterations are associated with a worse survival.The protein encoded byTRAF3can inhibit the activation of nuclear factor-κB and the mitogen-activated protein kinase signaling pathway.Moreover,it positively regulates the host innate immune response and T cell-dependent immune response,which may explain the specific infiltration of immunocytes observed in lung LELC.Of the cases,19.5% (8/41 cases)showed amplification of theCD247gene,which encodes the PD-L1 protein.This finding may be the dominant indicator for the use of anti-PD-1/PD-L1 therapy in the treatment of lung LELC[25].

Owing to the low incidence of lung LELC,there is a lack of large-sample studies.Therefore,other therapeutic regimens against NSCLC and nasopharyngeal carcinoma were used as references.It is generally accepted that patients with early stage disease mainly undergo radical surgery,while those with locally advanced or advanced disease adopt the multi-disciplinary comprehensive treatment strategy (i.e.,surgery,chemotherapy,and radiotherapy).There are numerous chemotherapeutic regimens.Third-generation chemotherapeutic drugs,such as paclitaxel,gemcitabine,pemetrexed,and vinorelbine combined with platinum,fluorouracil,and capecitabine,are commonly used regimens.Hanet al[26]reported five patients with LELC who underwent surgical resection of the lungs.Among them,the disease-free survival(DFS) in three patients was 5 years,and two patients had an overall survival of 45 months and 38 months.Fluorouracil combined with cisplatin was administered to seven patients with advanced lung LELC.The clinical partial remission rate was 71.4%[27].After progression while receiving first-line therapy,monotherapy with capecitabine was effective as a rescue regimen[28].In this case,although the initial EP regimen was effective,the remission time was short,suggesting that lung LELC may be more inclined to NSCLC rather than SCLC.This highlighted the need for additional experience to improve the treatment of similar cases.

The efficacy of tyrosine kinase inhibitors remains uncertain.It was reported that one case with anEGFR21 exon L858R mutation progressed 1 mo after treatment with a tyrosine kinase inhibitor[29].A study conducted in Taiwan found that the rate ofEGFRmutation in primary lung LELC was only 12.1% (8/66 cases),and there were no abnormalities in theALKandROS1genes[30].In recent years,immunological checkpoint inhibitors have become a hot topic in the treatment of NSCLC.The positive rate of PD-L1 expression in patients with lung LELC is between 63.3% (50/79 cases) and 74.3% (84/113 cases)[31,32].

Comprehensive treatment in patients with high expression of PD-L1 was more effective than in those with low expression in terms of progression-free survival and overall survival (P= 0.019 andP= 0.042,respectively)[33].However,postoperative DFS was worse in patients with high expression of PD-L1 than in those with low expression (5-year DFS:48.3%vs61.2%,respectively,P= 0.008)[32].In 2016,the Cancer Research Center of the American Cancer Institute published its first report on the treatment of advanced lung LELC with nivolumab (3 mg/kg,every 2 wk)[34].However,10 days after the first administration of the drug,patients developed treatment-associated pneumonia,and liver and lung metastases progressed.The patients received medication for the second time to exclude pseudo-progression;however,the patients expired due to immune-associated enteritis and persistent progression of the lesions.This suggests extensive heterogeneity in the immune function of patients with primary lung LELC,which requires further investigation.

The prognosis of lung LELC is generally better than that of the other types of NSCLC.Early stage of disease,absence of lymph node metastasis,complete resection,and normal levels of lactate dehydrogenase and albumin are good prognostic factors.In contrast,age ＞ 65 years is associated with a poor prognosis (hazard ratio:2.685,95% confidence interval:1.052-6.853,P= 0.039)[10,35].

CONCLUSION

We report the diagnosis and therapy of a patient with CD56-positive lung LELC,and performed a literature review regarding this rare type of tumor.The diagnosis of primary lung LELC was reached through imaging examination,lymph node biopsy,and IHC analysis.Although the tumor exhibited positivity for CD56,LCNEC was excluded based on the expression of EBER and the diagnostic criteria of the disease.The molecular mechanism of diffuse positive expression of CD56 warrants further investigation.