Chidamide based combination regimen for treatment of monomorphic epitheliotropic

Ting-Zhi Liu,Yi-Jia Zheng,Zhan-Wen Zhang,Shan-Shan Li,Jiao-Ting Chen,Ai-Hua Peng,Ren-Wei Huang

Ting-Zhi Liu,Yi-Jia Zheng,Jiao-Ting Chen,Ai-Hua Peng,Ren-Wei Huang,Department of Hematology,The Sixth Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510655,Guangdong Province,China

Zhan-Wen Zhang,Department of Nuclear Medicine,The Sixth Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510655,Guangdong Province,China

Shan-Shan Li,Department of Oncology,The Sixth Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510655,Guangdong Province,China

Abstract

Key words: Monomorphic epitheliotropic intestinal T cell lymphoma;Histone deacetylase inhibitor;Chidamide;Intensive chemotherapy;Stem cell transplantation;Case report

INTRODUCTION

Enteropathy-associated T cell lymphoma (EATL) is a rare and aggressive type of peripheral T cell lymphoma.It arises from T lymphocytes residing in the intraepithelial space of the intestine[1].It represents 10%-25% of primary gastrointestinal lymphomas and 5%-8% of all T-cell non-Hodgkin lymphomas[2].It was classified into two types (type I and type II) according to the World Health Organization (WHO)(2008) classification based on disparities in the epidemiologic and clinicopathological features[3].EATL type II,which comprises 10% to 20% of all EATL cases,was reclassified as monomorphic epitheliotropic intestinal T cell lymphoma (MEITL)according to the WHO (2016) classification[4].It appears to occur sporadically in Asian countries where the incidence of celiac disease is low[5].

The prognosis of MEITL has been considered poor with a median survival of 7 mo after diagnosis[4,6].Owing to the rarity and geographical variation of the disease,prospective and randomized clinical trials are difficult to be carried out in order to evaluate new treatment regimens incorporating novel agents.As a result,there are no validated and standardized treatment protocols for MEITL.Previous experience obtained favorable outcomes with combination chemotherapy followed by autologous hematopoietic stem cell transplant in patients who can tolerate such administration[5].Nevertheless,the overall prognosis is still poor.Additionally,many patients are unable to tolerate the toxicities of intensive therapy.Further studies are needed to explore effective novel regimens that can improve outcomes in this rare disease entity.Chidamide is one of the five histone deacetylase inhibitors (HDACIs)which has been approved in China[7].It is a novel orally active benzamide-type histone deacetylase inhibitor.Outcomes from phase I and phase II trials showed preferential efficacy in mature T-cell lymphoma[8].The efficacy of chidamide in MEITL has not been reported yet.

In this paper,we present two patients with MEITL who were given chidamide combined with chemotherapy after radical surgery.We also discuss the histopathological characteristics,diagnosis,and treatment strategies of MEITL in this paper.

CASE PRESENTATION

Chief complaints

Case 1:Upper abdominal pain and intermittent black stool for 2 mo and up to 15 kg of weight lost.

Case 2:Abdominal distention over a period of 1 mo.

Personal and family history

None.

Physical examination upon admission

Case 1:No special physical signs were found at the first visit.

Case 2:Physical examination showed no special signs.

Laboratory examinations

Case 1:Pathologic specimens demonstrated diffuse infiltration of medium-sized tumor cells in the small intestine.The karyotype was slightly irregular with visible nucleoli.The cytoplasm was eosinophilic or transparent.The tumor cells were diffusely distributed and infiltrated the whole intestinal wall.Necrosis and vascular invasion were also observed.Immunohistochemistry revealed that the neoplastic cells displayed CD56+,TIA-1+,Granzyme B+,CD3+,CD79a-,CD20-,CD4-,CD8-,CD2-,Perforin-,CD5-,EBERS-,and a high proliferation index (Ki67 index of 80%).A bone marrow aspirate showed normal cellularity and absence of neoplastic cells.

Case 2:Stool examination revealed occult blood.An immunohistochemical test showed that the tumor was positive for TIA-1,Granzyme B,CD3,and CD56,and negative for Perforin,CD20,and CD5.EBER was negative.Additionally,approximately 60% of the tumor cells were Ki-67 positive.Staging bone marrow examination showed no infiltration.

Imaging examinations

Case 1:A Positron emission tomography/computed tomography (PET/CT) scan revealed that the wall of the second group of the intestine was thickened with increased metabolism.Multiple enlarged and hypermetabolic lymph nodes in the para-intestinal,mesenteric,and retroperitoneal regions were shown (Figure 1).

Case 2:PET/CT demonstrated wall thickening of groups 1 and 4 of the small intestine and upper sigmoid colon with diffuse uptake.Furthermore,small lymph nodes in the para-intestinal and mesenteric regions were also metabolically active (Figure 2).

FINAL DIAGNOSIS

Case 1

The diagnosis was consistent with MEITL.According to the new EATL prognostic index (EPI),he was defined as intermediate risk[9].

Case 2

MEITL infiltrating the whole intestinal wall of the jejunum,small intestine,and sigmoid colon was demonstrated by histologic examination.Sixteen mesenteric lymph nodes were resected with no tumor involvement.She was also considered intermediate risk according to EPI.

TREATMENT

Case 1

A partial excision of the small intestine was performed to remove the tumor.After surgery,two cycles of CHOP (cyclophosphamide,doxorubicin,vincristine,and prednisolone) combined with chidamide were offered.Treatment was subsequently escalated to CHOEP (CHOP and etoposide) combined with chidamide for four cycles considering the malignant characteristic of this disease.

Case 2

Figure1 Pre-operative positron emission tomography/computed tomography of case one.

On day 4 after admission,she felt severe abdominal pain and high fever (her highest temperature was 39.2 °C).Abdominal plain film indicated intestinal perforation.On the fifth day,her blood pressure decreased to 79/49 mmHg,and septic shock was considered.Emergency laparotomy was performed.A perforation occurred in the sigmoid colon was found.Eventually,sigmoid colostomy was executed.After surgery,she received combination chemotherapy with a regimen of IVE (ifosfamide,etoposide,and vincristine) and chidamide for six cycles.

OUTCOME AND FOLLOW-UP

Case 1

In the middle of chemotherapy,a PET/CT re-evaluation showed no evidence of abnormal hypermetabolic lesion,which confirmed a complete remission.The patient decided to discontinue chemotherapy because of his fragile constitution.Eleven months following his diagnosis,he complained of recurrent cough and sputum.Antiinfection and diagnostic anti-tuberculosis treatment were given at a local hospital without benefit.He returned to our hospital.An ultrasound-guided percutaneous lung biopsy in the left upper lung confirmed the recurrence of MEITL.He underwent three cycles of C-PCT regimen (chidamide plus prednisone,cyclophosphamide,and thalidomide) as rescue therapy.During this time,myelosuppression and pneumonia repeatedly occurred,accompanied by dizziness,fatigue,night sweat,sore throat,cough,sputum,hoarseness,chest tightness,and shortness of breath after exercise.His general condition eventually deteriorated.A CT scan showed that the shadow in the bilateral lungs was aggravating (Figure 3).Enlarged lymph nodes in the mediastinal and right hilar were also slightly larger.On October 20,15 mo after his diagnosis,the patient decided to quit all treatment.He then left the hospital and died a few days later.

Case 2

A PET/CT re-evaluation was performed.No hypermetabolic lesion was seen.The efficacy was evaluated as CR.Six months following her diagnosis,she developed diplopia without dizziness,headache,and nausea and vomiting.An enhanced sinus/nasopharynx and head magnetic resonance imaging (MRI) scan revealed multiple lesions in the bilateral frontotemporal lobes,semioval centers,basal ganglia,insular lobes,left hippocampus,and cerebral foot,which were considered to be lymphoma infiltration.High-dose methotrexate (3.5 g/m2) combined with temozolomide (200 mg d1-3) and chidamide as well as intermittent lumbar puncture and intrathecal injection of methotrexate,cytorabine,and dexamethasone were given as rescue therapy.After two cycles,a head MR evaluation showed that the lesions in the bilateral fronto-parietal temporal lobes,semioval center,basal ganglia,insular lobes,the left hippocampus,and cerebral foot shrank slightly (Figure 4).Unfortunately,afterwards,the lesions aggravated gradually.The patient passed away 17 mo after her diagnosis.

Figure2 Pre-operative positron emission tomography/computed tomography of case two.

DISCUSSION

EATL is a kind of rare disease which arises from the intraepithelial T-lymphocytes of intestine[1].Originally,it was classified into EATL types I and II in the WHO (2008)classification[3].A better understanding of disease biology led to a change in the terminology.EATL type I is now designated as EATL[1].It is closely associated with refractory celiac disease,characterized by an allergic reaction to gluten,and associated with human leukocyte antigen class II haplotypes human leukocyte antigen-DQ2 or DQ8[10].EATL occurs more frequently in areas with a high prevalence of celiac disease,especially in Northern Europe and America[11].It is rare in East Asian because of the rarity of celiac disease[11].Although there are many similarities in the clinicopathological features of EATL and MEITL,some distinct histological,immunophenotypical,and molecular markers exist[5].Histologically,the neoplastic cells of MEITL are mainly monotonous and small to intermediate in size,with round to slightly irregular nuclei,small inconspicuous nucleoli,and scant cytoplasm.The growth pattern is transmural and prone to ulceration,particularly frequent in areas of perforation.The adjacent mucosa contains heavy epitheliotropism without associated inflammatory background.Immunohistochemistry showed activated cytotoxic phenotype including CD3+,CD7+,CD5-,CD4-,CD30-,CD8+,and CD56+.Sometimes,CD8 or CD56 is negative[5].

Due to the tumor location and clinical presentation,many patients (＞ 80%) need surgical resection at the time of presentation[4,12,13].Surgery is performed as an emergent procedure in a proportion of patients (＞ 40%) due to obstruction and/or perforation[12].According to research,small bowel aggressive B cell lymphoma and Tcell lymphomas are high risk factors contributing to perforation.Patients who had perforation and received emergency surgery had a worse survival[14,15].In our study,the male patient received resection designedly to remove the tumor,and he presented with recurrence 11 mo after his diagnosis.The female patient was administered an emergent abdominal exploration owing to intestine perforation.Only 6 mo later,bilateral brain recurrence was found.

Figure3 Thoracic computed tomography scan of case one taken at recurrence 11 mo following his diagnosis and re-evaluation after three cycles of rescue chemotherapy.

The course of MEITL is very aggressive.Prior research reported a dismal 5-year survival of approximately 20%[2].De Baaijet al[9]developed a new prognostic model termed EATL prognostic index (EPI) to distinguish patients into three risk group based on B symptoms and international prognostic index score.The median overall survival was 2,7,and 34 mo for patients with low,intermediate,and high risk,respectively[9].In our study,both patients were defined as intermediate risk according to EPI.Historically,after surgical intervention,anthracycline-based regimens including CHOP or CHOPE with or without consolidative autologous stem cell transplantation (ASCT) was recommended to improve the prognosis[2,4,6,12,16].However,while anthracycline based regimens led to an overall response rate of 36%-58%,17%-38% of patients had refractory disease.Most of those subjects relapsed eventually[2,4,12].

Figure4 Head magnetic resonance imaging of case two taken at recurrence 6 mo following her diagnosis and re-evaluation after two cycles of rescue therapy.

High-dose chemotherapy with autologous stem cell transplantation was reported to have promising results in a few case reports and small series.In a prospective trial conducted by The Scotland and Newcastle Lymphoma Group[12],26 EATL patients were treated with induction chemotherapy with a regimen of IVE/MTX (ifosfamide,vincristine,and etoposide,alternated with methotrexate) followed by ASCT.It indicated that the regimen obtained a superior overall response rate with an acceptable safety profile compared to historical controls treated with anthracyclinebased regimens (69%vs42%).The 5-year progression-free survival and overall survival rates were 52% and 60%,respectively.Disease progression,therapy-related side effects,and declining performance status led to failure to complete therapy.

Although an improved survival has been obtained after aggressive consolidation therapy,the true efficacy of those modalities and side effects remain to be identified.Due to comorbidities,performance status,and fragile state of patients following total colectomy,only 50% of patients were reported to be eligible to undergo planned chemotherapy[12].In our study,the male patient aged 61 years had no special medical history,however,he quitted therapy early due to intolerability of side effects of chemotherapy.This indicated that elderly patients might be unable to endure intensive chemotherapy especially after small bowl resection which can deteriorate the nutrition status.

Development of newer treatment paradigms integrating targeted drugs such as brentuximab vedotin (an anti-CD30 conjugated antibody) is essential to improve outcomes.Khalafet al[17]reported on a patient with EATL successfully treated with brentuximab vedotin as salvage therapy who had a good response and disease remission at 9 mo of follow-up.Additional case reports using alemtuzumab (a monoclonal antibody against CD52)[18,19]and romidepsin[20](an HDACI) have shown promising clinical responses.

Chidamide is one of five HDACIs (the other four are belinostat,vorinostat,panobinostat,and romidepsin) which have been approved in China[7].It showed favorable efficacy in mature T-cell lymphoma in phase I trials[8].In a phase II trial,an overall response rate of 28% was achieved in 79 patients with relapsed or refractory mature T-cell lymphomas with a median duration of response of 9.9 (1.1-40.8) mo[8].

The patients in our study were both intermediate risk.In order to improve the efficacy of chemotherapy,strategies including chidamide combined with chemotherapy following radical surgery were given.They developed recurrence 11 and 6 mo after the diagnosis and obtained an overall survival of 15 and 17 mo,respectively,which were slightly longer than outcomes in previous studies (7 mo)[9].This indicated the poor prognosis of this disease entity and chidamide can be used as a novel strategy to improve the prognosis.

Additionally,in our study,the patients were assigned to PET/CT scans to reveal gastrointestinal tumor involvement.The lymphoid cells were metabolically active,as detected in PET/CT,indicating their aggressive characteristics.We recommend PET/CT as a useful tool which can aid early detection and staging of EATL.A previous analysis also showed a higher potential of PET scan to identify EATL compared to CT scan (100%vs87%)[21].

Pathological examination found no tumor cells in lymph nodes from the periintestinal region,suggesting tumor infiltration of the gastrointestinal region specifically.However,recurrence sites of both cases were found to be distant,such as the lung and brain.This implies that the tumor cells disseminate hematogenously,and their metastasis is not prevented by intensive chemotherapy.

CONCLUSION

In summary,we report two cases of MEITL treated with chidamide combined with chemotherapy with slightly improved survival time.Based on our experience and previous studies,we concluded that the high mortality of MEITL is associated with rapid tumor growth,low chemo-sensitivity,and a tendency to disseminate.The poor condition of patients due to prolonged and severe malnutrition compromises the ability to tolerate chemotherapy.To improve the prognosis,a multimodality treatment approach is suggested in eligible MEITL patients.Surgical de-bulking is preferably the primary step as early as possible followed by subsequent intensive chemotherapy,though the optimal choice of regimen remains to be defined.Consolidation ASCT can be considered in available patients due to its superior outcomes obtained in EATL with the majority of patients lacking marrow infiltration.Novel targeted pharmaceuticals may play a role in this type of lymphoma.However,further investigations are warranted.

ACKNOWLEDGEMENTS

We thank Dr.Rong-Ni He for his kind assistance.