miRNA—1和心肺复苏后心功能障碍严重程度的相关性研究

戴备军　张美齐　翁剑枫　胡志斌

[摘要] 目的 探討miRNA-1表达水平和心肺复苏后心功能障碍严重程度的相关性。 方法 选择2014年2月～2016年2月期间在本院急诊科心肺复苏成功的心脏骤停患者62例作为病例组，按年龄、性别匹配取62例健康自愿者作为对照组，采用Real-time PCR检测miRNA-1表达水平。观察病例组患者超声心动图心功能各项指标，分析miRNA-1表达水平与心功能障碍严重程度的相关性。 结果 病例组miRNA-1表达水平明显高于对照组（P<0.05），心功能Ⅳ级组miRNA-1表达水平明显高于心功能Ⅲ级组（P<0.05），miRNA-1表达水平与心功能指标E峰、E/A值、LVEF、LVEDD呈负相关（P<0.05），miRNA-1表达水平与心功能障碍严重程度呈正相关（r=0.864， P<0.01）。 结论 miRNA-1参与了心肺复苏后心肌细胞凋亡的发生和发展，与心功能障碍严重程度密切相关，其具体途径可能是通过调节靶基因从而促进或抑制靶基因蛋白表达而实现。

[关键词] 心肺复苏术；miRNA-1；心功能障碍；严重程度

[中图分类号] R605.974 [文献标识码] A [文章编号] 1673-9701（2017）11-0005-03

Correlation study of miRNA-1 and severity of cardiac dysfunction after cardiopulmonary resuscitation

DAI Beijun1 ZHANG Meiqi2 WENG Jianfeng3 HU Zhibin1

1.Department of Cardiothoracic Surgery，Zhejiang Provincal Peoples Hospital， Hangzhou 310014， China；2.Department of Emergency，Zhejiang Peoples Hospital， Hangzhou 310014， China；3.Zhejiang University of Science and Technology， Hangzhou 310012，China

[Abstract] Objective To investigate the correlation between the expression level of miRNA-1 and the severity of cardiac dysfunction after cardiopulmonary resuscitation. Methods 62 patients with cardiac arrest who were given cardiopulmonary resuscitation in the Emergency Department in our hospital from February 2014 to February 2016 were assigned to the subject group. 62 healthy volunteers were assigned to the control group by age and gender. The expression level of miRNA-1 was tested by real-time PCR. The indices of cardiac function were observed in the subject group by echocardiography， and the correlation between the expression level of miRNA-1 and the severity of cardiac dysfunction was analyzed. Results The expression level of miRNA-1 in the subject group was significantly higher than that in the control group （P<0.05）. The expression level of miRNA-1 in the cardiac function level Ⅳ group was significantly higher than that in the cardiac function level Ⅲ group（P<0.05）. The expression level of miRNA-1 was negatively correlated with E-peak， E/A value， LVEF and LVEDD（P<0.05）. The expression level of miRNA-1 was positively correlated with the severity of cardiac dysfunction（r=0.864， P<0.01）. Conclusion miRNA-1 participates in the occurrence and progression of myocardial apoptosis after cardiopulmonary resuscitation， and it is closely related to the severity of cardiac dysfunction. The specific pathway may be obtained by modulating the target gene in order to promote or inhibit the expression of the target gene protein.